Isolation and characterization of trehalose tetraester biosurfactants from a soil strain Micrococcus luteus BN56 |
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Authors: | Borjana Tuleva Nelly Christova Rashel Cohen Daniela Antonova Tihomir Todorov Ivanka Stoineva |
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Institution: | 1. Institute of Microbiology, Bulgarian Academy of Sciences, Acad.G.Bonchev str, bl 26, 1113 Sofia, Bulgaria;2. Institute of Physical Chemistry, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria;3. Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria;4. Genetic Medico-Diagnostic Laboratory “Genica”, 1463 Sofia, Bulgaria |
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Abstract: | The bacterium Micrococcus luteus BN56, isolated from soil, was found to produce glycolipid biosurfactants when grown on n-hexadecane as the sole carbon source. The purified glycolipids were characterized using 1H, 13C, 1H COSY NMR-spectroscopy and ESI-MS spectrometry analyses. The two main products were identified as trehalose tetraesters with molecular mass of 876 and 848 g mol?1. The purified products reduced the surface tension of water from 72 to 24.1 mN m?1 and the interfacial tension between water and hexadecane from 43.0 to 1.7 mN m?1. The CMC of these biosurfactants was found to be 25 mg l?1. The strain formed stable emulsions with hydrocarbon substrates and was suggested that the hydrophobic cells acted as emulsion-stabilizing agents. The results demonstrate that the strain M. luteus BN56 may be well suited for bioremediation of oil-contaminated environments. |
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