Enzymatic solubilization of a pectinaceous dietary fiber fraction from potato pulp: Optimization of the fiber extraction process |
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| Authors: | Anne S Meyer Birgitte P Dam Helle N Lærke |
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| Institution: | 1. Center for BioProcess Engineering, Department of Chemical and Biochemical Engineering, Building 229, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark;2. Department of Animal Health, Welfare and Nutrition, Faculty of Agricultural Sciences, University of Aarhus, DK-8830 Tjele, Denmark;1. Department of Materials Engineering, Faculty of Engineering, Kasetsart University, 50 Ngamwongwan Rd., Chatuchak, Bangkok, 10900, Thailand;2. Interdisciplinary Graduate Program in Advanced and Sustainable Environmental Engineering (International Program), Faculty of Engineering, Kasetsart University, 50 Ngamwongwan Rd., Chatuchak, Bangkok 10900, Thailand;3. Department of Environmental Technology and Management, Faculty of Environment, Kasetsart University, 50 Ngamwongwan Rd., Chatuchak, Bangkok, 10900, Thailand;4. Centre of Excellence on enVironmental Strategy for GREEN Business (VGREEN), Faculty of Environment, Kasetsart University, 50 Ngamwongwan Rd., Chatuchak, Bangkok 10900, Thailand;5. Materials Innovation Center, Faculty of Engineering, Kasetsart University, 50 Ngamwongwan Rd., Chatuchak, Bangkok, 10900, Thailand;1. Laboratory Biocomposite Technology, Institute of Tropical Forestry and Forest Products, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Darul Ehsan, Malaysia;2. Forest Product Division, Forest Research Institute Malaysia (FRIM), 52109 Kepong Selangor, Daurl Ehsan, Malaysia;3. Advanced Materials and Nanotechnology Lab., Institute of Advanced Technology, Universiti Putra Malaysia,43400 UPM Serdang, Selangor, Malaysia;1. College of Food Science, Shanxi Normal University, Linfen 041004, China;2. College of Life Sciences and Food Engineering of Hebei Engineering University, Handan 056038, China;1. Department of Technique and Food Development, Warsaw University of Life Sciences, 02-776, Warsaw, Poland;2. Institute of Food and Nutriceutical Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Dongchuan Road 800, Shanghai, 200240, China |
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| Abstract: | Upgrading of potato pulp, a byproduct stream from industrial manufacture of potato starch, is important for the continued economic competitiveness of the potato starch industry. The major part of potato pulp consists of the tuber plant cell wall material which is particularly rich in galactan branched rhamnogalacturonan I type pectin. In the work reported here, the release of high-molecular weight pectinaceous dietary fiber polysaccharides from starch free potato pulp was accomplished by use of a multicomponent pectinase preparation from Aspergillus aculeatus (Viscozyme® L). The enzyme reaction conditions for the solubilization were optimized via a surface response design to be addition of 0.27% Viscozyme® L by weight of potato pulp substrate dry matter, 1 h treatment at pH 3.5, 62.5 °C. Analysis of the molecular size and monomer composition of the enzymatically released fibers showed that they were rich in galactose and uronic acid indicating that the solubilized fibers were mainly made up of galactan branched rhamnogalacturonan I type pectin polymers. |
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