A new ginsenosidase from Aspergillus strain hydrolyzing 20-O-multi-glycoside of PPD ginsenoside |
| |
| Authors: | Hongshan Yu Qingmei Liu Chunzhi Zhang Mingchun Lu Yaoyao Fu Wan-Taek Im Sung-Taik Lee Fengxie Jin |
| |
| Institution: | 1. College of Bio & Food Technology, Dalian Polytechnic University, Qinggong-yuan No. 1, Ganjingzi-qu, Dalian 116034, People''s Republic of China;2. Environmental and Molecular Microbiology Lab., Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, South Korea |
| |
| Abstract: | A ginsenosidase specifically hydrolyzing multi-20-O-glycosides of protopanaxadiol type ginsenosides such as ginsenoside Rb1, Rb3, Rb2 and Rc, named ginsenosidase type II, was isolated and purified from Aspergillus sp.g48p strain. The molecular weight of the enzyme was 60 kDa. Ginsenosidase type II was demonstrated to hydrolyze multi-20-O-glycoside of protopanaxadiol type ginsenoside Rb1, Rb3, Rb2 and Rc; i.e. the ginsenosidase type II hydrolyzes 20-O-β-glucoside of the ginsenoside Rb1, 20-O-β-xyloside of ginsenoside Rb3, 20-O-α-arabinoside(p) of ginsenoside Rb2 and α-arabinoside(f) of ginsenoside Rc to produce mainly ginsenoside Rd, and small amount of Rg3. However, it did not hydrolyze 3-O-β-glucosides of ginsenoside Rb1, Rb3, Rb2 and Rc which was different with the ginsenosidase type I previously reported, either did not hydrolyze the glycosides of protopanaxatriol type ginsenoside such as ginsenoside Re, Rf and Rg1, showing significant difference from all previously described glycosidases. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
