Enhanced biotransformation of l-phenylalanine to 2-phenylethanol using an in situ product adsorption technique |
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Authors: | Jianfeng Mei Hang Min Zhenmei Lü |
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Affiliation: | 1. College of Life Science, Zhejiang University, Hangzhou 310058, China;2. College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310032, China |
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Abstract: | An in situ product adsorption technique was used to enhance the biotransformation of l-phenylalanine to 2-phenylethanol by Saccharomyces cerevisiae BD. As a suitable adsorbent, the non-polar macroporous resin D101, selected from several resins tested, showed high adsorption capacity for 2-phenylethanol but not l-phenylalanine. Product inhibition was effectively alleviated by the addition of macroporous resin D101 to the biotransformation medium. When 2 g of hydrated resin D101 was added to 30 mL of the biotransformation medium, the total 2-phenylethanol concentration achieved was 6.17 g/L, of which 3.15 g/L remained in the aqueous phase and 3.02 g/L was adsorbed onto the resin. The molar yield of 2-phenylethanol reached 0.70 after 24 h cultivation. Addition of the macroporous resin greatly increased the volumetric productivity of 2-phenylethanol, and made the downstream processing more feasible and easier to perform in an industrial application. |
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