Localization of a Baculovirus-Induced Chitinase in the Insect Cell Endoplasmic Reticulum |
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| Authors: | Carole J Thomas Helen L Brown Chris R Hawes Bum Yong Lee Mi-Kyung Min Linda A King and Robert D Possee |
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| Institution: | NERC Institute of Virology and Environmental Microbiology, Oxford OX1 3SR,1. and School of Biological and Molecular Sciences, Oxford Brookes University, Oxford OX3 0BP,2. United Kingdom, and MOGAM Biotechnology Research Institute, Yongin-Kun, Kyonggi-Do 449-910, Korea3. |
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| Abstract: | Confocal immunofluorescence microscopy was used to demonstrate that the Autographa californica nucleopolyhedrovirus (AcMNPV) chitinase was localized within the endoplasmic reticulum (ER) of virus-infected insect cells. This was consistent with removal of the signal peptide from the chitinase and an ER localization motif (KDEL) at the carboxyl end of the protein. Chitinase release from cells, a prerequisite for liquefaction of virus-infected insect larvae, appears to be aided by synthesis of the p10 protein. Deletion of p10 from the AcMNPV genome delayed the appearance of chitinase activity in the medium of virus-infected cells by 24 h and also delayed liquefaction of virus-infected Trichoplusia ni larvae by the same period. |
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