Physical and chemical characterization of two- and three-stranded adenine-thymine and adenine-uracil homopolymer complexes |
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| Authors: | M Riley B Maling |
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| Affiliation: | 1. Department of Basic Chemistry, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian Province 350108, PR China;2. Department of Pharmaceutical Analysis, The higher educational key laboratory for Nano Biomedical Technology of Fujian Province, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian Province 350108, PR China |
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| Abstract: | The DNA homopolymers dA and dT have been prepared enzymically using Escherichia coli DNA polymerase, and their properties have been studied. Mixtures of dT4 polymer with dA or rA polymers reacted rapidly to form the homopolymer pairs dA:dT and rA:dT. These complexes resembled the RNA pair rA:rU in being two-stranded helical structures containing complementary base pairs; however, the three homopolymer pairs differ in thermal stability. The homopolymer pair dA:rU is not stable under any conditions of temperature and salt concentration tested; mixtures of dA with rU contain three-stranded dA:rU+2 as the only helical complex. The other AT and AU homopolymer pairs can also form three-stranded complexes containing an additional equivalent of either dT or rU polymer under certain conditions. |
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