Polyol metabolism in the mycelium and fruit-bodies during development ofFlammulina velutipes |
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Authors: | Yukata Kitamoto Atsuko Kikuchi Nobuhiro Mori Shoji Ohga |
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Institution: | (1) Department of Biochemistry and Biotechnology, Faculty of Agriculture, Tottori University, 4-101, Minami, Koyama, 680-0945 Tottori, Japan;(2) Research Institute of University Forests, Faculty of Agriculture, Kyushu University, Tubakuro 394, Sasaguri, 811-2415 Fukuoka, Japan |
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Abstract: | Changes of polyol contents in the mycelium and fruit-bodies ofFlammulina velutipes were measured. The results suggested that arabinitol is accumulated in the fruit-bodies as the end-product after its translocation
from the mycelium, while mannitol in the fruit-bodies is converted into fructose by the action of mannitol dehydrogenase (MDH).
The development of fruit-bodies was promoted by feeding of mannitol to the mycelial colony. A14C tracer experiment indicated that half of mannitol translocated from mycelium to fruit-bodies was utilized for fruit-body
development. NAD-linked MDH andd-arabinitol dehydroganase (D-ADH) were detected in both mycelium and fruit-bodies. The activities of MDH and ADH in the mycelium
reached their maximum levels in the inital stage of fruit-body development and decreased thereafter. In contrast, the activity
of MDH in the fruit-bodies showed a peak in the middle stage of development. The activity of ADH in the fruit-bodies was less
than half of that of MDH. MDH showed a lower Km value for mannitol (1.3 ×10−3M) than for fructose (6.0×10−2 M). The Km value of ADH for arabinitol was extremely high (1.3×10−1M). |
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Keywords: | arabinitol dehydrogenase Flammulina velutipes fruit-body mannitol mannitol dehydrogenase |
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