首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Polyol metabolism in the mycelium and fruit-bodies during development ofFlammulina velutipes
Authors:Yukata Kitamoto  Atsuko Kikuchi  Nobuhiro Mori  Shoji Ohga
Institution:(1) Department of Biochemistry and Biotechnology, Faculty of Agriculture, Tottori University, 4-101, Minami, Koyama, 680-0945 Tottori, Japan;(2) Research Institute of University Forests, Faculty of Agriculture, Kyushu University, Tubakuro 394, Sasaguri, 811-2415 Fukuoka, Japan
Abstract:Changes of polyol contents in the mycelium and fruit-bodies ofFlammulina velutipes were measured. The results suggested that arabinitol is accumulated in the fruit-bodies as the end-product after its translocation from the mycelium, while mannitol in the fruit-bodies is converted into fructose by the action of mannitol dehydrogenase (MDH). The development of fruit-bodies was promoted by feeding of mannitol to the mycelial colony. A14C tracer experiment indicated that half of mannitol translocated from mycelium to fruit-bodies was utilized for fruit-body development. NAD-linked MDH andd-arabinitol dehydroganase (D-ADH) were detected in both mycelium and fruit-bodies. The activities of MDH and ADH in the mycelium reached their maximum levels in the inital stage of fruit-body development and decreased thereafter. In contrast, the activity of MDH in the fruit-bodies showed a peak in the middle stage of development. The activity of ADH in the fruit-bodies was less than half of that of MDH. MDH showed a lower Km value for mannitol (1.3 ×10−3M) than for fructose (6.0×10−2 M). The Km value of ADH for arabinitol was extremely high (1.3×10−1M).
Keywords:arabinitol dehydrogenase            Flammulina velutipes            fruit-body  mannitol  mannitol dehydrogenase
本文献已被 ScienceDirect SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号