A luxCDABE-based bioluminescent bioreporter for the detection of phenol |
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| Authors: | Abd-El-Haleem D Ripp S Scott C Sayler G S |
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| Institution: | (1) The Center for Environmental Biotechnology, The University of Tennessee, Knoxville, TN 37996, USA, US;(2) The Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), Mubarak City for Scientific Research and Technology Applications, New Burg-Elarab City, Alexandria, Egypt, EG |
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| Abstract: | A bioluminescent reporter strain, Acinetobacter sp. DF4-8, was constructed for the detection of phenol by inserting a mopR-like promoter upstream of the Vibrio fischeri bioluminescent luxCDABE gene cassette in a modified mini-Tn5 construct. When introduced into the chromosome of Acinetobacter sp. DF4, the bioreporter produced a sensitive bioluminescent response to phenol at concentrations ranging from 2.5 to 100
ppm. This response was linear (R
2=0.986) in the range from 20 to 90 ppm. A significant bioluminescent response was also recorded when strain DF4-8 was incubated
with slurries from aged, phenol-contaminated soil.
Received 13 February 2002/ Accepted in revised form 11 July 2002 |
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| Keywords: | : Acinetobacter bioluminescence bioreporter lux mopR phenol |
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