Over-stabilization of Candida antarctica lipase B by ionic liquids in ester synthesis |
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Authors: | P Lozano T De Diego D Carrié Michel Vaultier JL Iborra |
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Institution: | (1) Departamento de Bioquímica y Biología Molecular B e Inmunología, Facultad de Química, Universidad de Murcia, P.O. Box, 4021, E-30100 Murcia, Spain;(2) Université de Rennes 1, Institut de Chimie, UMR CNRS 6510, Campus de Beaulieu, Av. Général Leclerc, 35042 Rennes, France |
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Abstract: | Four different ionic liquids, based on dialkylimidazolium cations associated with perfluorinated and bis(trifluoromethyl)sulfonyl amide anions were used as reaction media for butyl butyrate synthesis catalyzed by free Candida antarctica lipase B at 2% (v/v) water content and 50 °C. Lipase had enhanced synthetic activity in all ionic liquids in comparison with two organic solvents (hexane, and 1-butanol), the enhanced activity being related to the increase in polarity of ionic liquids. The continuous operation of lipase with all the assayed ionic liquids showed over-stabilization of the enzyme. The reuse of free lipase in 1-butyl-3-methylimidazolium hexafluorophosphate in continuous operation cycles showed a half-life time 2300 times greater than that observed when the enzyme was incubated in the absence of substrate (3.2 h), and a selectivity higher than 90%. |
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Keywords: | enzyme stability ionic liquids lipase non-conventional media synthesis |
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