Hydroxyl radical induced cross-linking between phenylalanine and 2-deoxyribose |
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Authors: | M Farahani M G Simic |
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Institution: | Center for Radiation Research, National Bureau of Standards, Gaithersburg, Maryland 20899. |
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Abstract: | Hydroxy radicals induce cross-linking between phenylalanine (Phe) and 2-deoxyribose (dR) via formation of corresponding free radical intermediates. The cross-linked products were separated and identified by capillary gas chromatography-mass spectrometry. When phenylalanine and 2-deoxyribose radicals were generated in a 1:1 ratio, the predominant interaction was between Phe and dR radicals while the Phe-Phe and dR-dR cross-links were less abundant. The newly discovered cross-link between 2-deoxyribose and phenylalanine may serve as a model for radiation or free radical induced cross-linking between DNA and proteins and in general between sugar moieties and amino acids. |
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