Quantitative analysis of cyclic AMP waves mediating aggregation in Dictyostelium discoideum |
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| Authors: | P N Devreotes M J Potel S A MacKay |
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| Institution: | 1. Department of Physiological Chemistry, Johns Hopkins University, 725 N. Wolfe Street, Baltimore, Maryland 21205 USA;2. Department of Biophysics and Theoretical Biology, University of Chicago, 920 E. 58th Street, Chicago, Illinois 60637 USA |
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| Abstract: | We have previously reported the detection of cAMP waves within monolayers of aggregating Dictyostelium discoideum cells (K. J. Tomchik and P.N. Devreotes, 1981, Science 212, 443-446). The computer-assisted analysis presented here of the fluorographic images of the cAMP waves reveals (1) all the waves have a consistent width and height; (2) cAMP concentrations within centers of concentric aggregation territories oscillate periodically while at spiral centers the concentration builds up to a plateau value within 2 mm; (3) cells within the region of intersection of two oppositely directed cAMP waves are stimulated to produce more cAMP than those responding to a single wave; (4) cells start to move when the cAMP level begins to increase and cease movement when the peak cAMP concentration reaches the cell. |
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| Keywords: | To whom all correspondence should be addressed |
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