A fungal chitinase gene fromRhizopus oligosporus confers antifungal activity to transgenic tobacco |
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Authors: | T Terakawa N Takaya H Horiuchi M Koike M Takagi |
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Institution: | (1) Central Research Laboratories, Hokko Chemical Ind. Co., 2165 Toda, 243 Atsugi-shi, Kanagawa, Japan;(2) Department of Biotechnology, The University of Tokyo, Yayoi, Bunkyo-ku, 113 Tokyo, Japan |
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Abstract: | We have studied whether a chitinase involved in cell autolysis of a filamentous fungus,Rhizopus oligosporus, can operate as an antifungal defense system in tobacco. Thechi1 gene was introduced into tobacco by theAgrobacterium tumefaciens leaf disc system. Among 22 transgenic tobacco plants, 2 were selected and their individual homozygous progeny, Tch1-1 and Tch2-1, were studied. Chitinase activity in the extracts of young leaves from Tch1-1 or Tch2-1, in which thechi1 gene product was detected by Western blot analysis, was three- to four-fold higher than that from the control plants. A fungal infection assay on the leaves infected with the discomycete pathogensSclerotinia sclerotiorum andBotrytis cinerea revealed that the symptoms observed with these two were remarkably suppressed as compared with the control leaves.Abbreviations
CaMV
Cauliflower mosaic virus
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Km
r
kanamycin resistant
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Km
s
kanamycin sensitive
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MS
Murashige and Skoog
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PCR
polymerase chain reaction
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PDA
potato dextrose agar
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PR
pathogenesis-related |
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Keywords: | Fungal chitinase Transgenic tobacco Antifungal activity |
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