Statistical optimization of medium components for the production of xylanase byAspergillus niger KK2 in submerged cultivation |
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Authors: | Byeong Jo Min Yang Soon Park Seong Woo Kang Yoon Seok Song Jong Ho Lee Chulhwan Park Chan Wha Kim Seung Wook Kim |
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Affiliation: | (1) College of Life Sciences and Biotechnology, Korea University, 136-701 Seoul, Korea;(2) CTCBIO Inc., 445-913 Hwaseong, Korea;(3) Department of Chemical and Biological Engineering, Korea University, 136-701 Seoul, Korea;(4) Department of Chemical Engineering, Kwangwoon University, 139-701 Seoul, Korea |
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Abstract: | Medium composition was optimized for the production of xylanase byAspergillus niger KK2 using statistical experimental designs. Corn steep liquor (CSL) and industrial yeast extract (IYE) were the most important factors affecting xylanase activity. The medium that produced the optimum conditions for the production of xylanase contained 3% rice straw, 1% wheat bran, 6.3% CSL, 0.15% IYE, and 0.5% KH2PO4. After 4 days of cultivation under optimized conditions in a 2.5-L stirred tank reactor the activity and productivity of xylanase were 620 IU/mL and 6,458 IU/L.h, respectively. The highest xylanase activity obtained using the optimized medium was 80% greater than the activity obtained using basal medium. The xylanase activity predicted by a polynomial model was 670 IU/ml. |
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Keywords: | Aspergillus niger corn steep liquor (CSL) response surface methodology (RSM) rice straw xylanase |
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