Generate Western blot protein marker from a single construct |
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Authors: | Yi-Ming Zhang Liang Geng Da-Wei Huang |
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Institution: | aInstitute of Zoology, Chinese Academy of Sciences, Beijing 100101, China;bGraduate School of the Chinese Academy of Sciences, Beijing 100039, China;cCollege of Plant Protection, Shandong Agricultural University, Tai’an, Shandong 271018, China |
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Abstract: | An expression construct, consisting of a tandem arrangement of nucleic acids coding for the constant fragments (Fc) receptor of protein G combined with nucleic acids for the Fc receptor of protein A, was constructed. When the construct was expressed in Escherichia coli, proteins of estimated molecular weights of 25, 30, 50, 58, 80, and 85 kDa were consistently obtained from this expression construct due to possible proteolytic degradation during the cultivation and purification steps. The purified proteins from this single expression construct were used as Western blot protein marker. |
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