Differential hydrolysis of 1-alkyl-2-acyl and diacylglycerophosphocholines by human and non-human phospholipases A2. |
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| Authors: | F Cabré A M García A Carabaza D Mauleón G Carganico |
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| Affiliation: | R & D Department, Laboratorios Menarini S.A., Badalona, Spain. |
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| Abstract: | The activity of two human phospholipases A2, purified from synovial fluid and lumbar disc herniations, was tested using alkylacyl- and diacylglycerophosphocholines and the influence of the chemical link at the sn-1 position of glycerol was investigated. Both enzymes exhibited 2.5-3-fold selectivity for 1-ester-linked compared to 1-ether-linked phosphatidylcholine. No significant selectivity was observed with pancreatic phospholipase A2 while Naja naja naja venom enzyme was more efficient against 1-ether-phospholipids. |
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