| Abstract: | The conversions of the isotope from 1-14C]acetate, 1-14C]glucose and 6-14C]glucose to CO2 and fatty acids in acini isolated from the mammary gland at the peak of lactation were studied. The incorporation of 9,10-3H]oleate into triacylglycerol synthesis as single substrate or in combination with substrates that potentially may supply trioses-phosphate was also determined. The rate of fatty acid synthesis paralleled the activity of the hexose monophosphate shunt and the data obtained reveal that little carbon from triose stage enters the phosphohexose pool via reversal of glycolytic pathway. The results are interpreted in terms of the NADPH producing systems and phosphoenolpyruvate carboxykinase activities as well as the possible implications in lipogenic and glyceroneogenic pathways. |
