Analysis of Leptospira isolates from mainland Portugal and the Azores islands |
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Authors: | Collares-Pereira M Korver H Cao Thi B V Santos-Reis M Bellenger E Baranton G Terpstra W J |
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Institution: | Instituto de Higiene e Medicina Tropical (UNL), R. da Junqueira 96, 1349-008, Lisboa, Portugal. mcp@ihmt.unl.pt |
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Abstract: | From 228 recent Leptospira isolates from mainland Portugal and Azorean wild mammals, 149 were characterized at the serovar level by monoclonal antibodies (MAbs), a quick serological method in epidemiological studies. In order to compare this antigenic information with that from new genetic techniques, a sample of isolates was analyzed through pulsed-field agarose gel electrophoresis (PFGE) (n=71), mapped restriction site polymorphisms (MRSPs) in PCR-amplified rRNA genes (n=45, including 13 saprophytes) and arbitrarily primed polymerase chain reaction (AP-PCR) fingerprinting (n=32). MRSP and AP-PCR lead to species identification of the studied 32 pathogenic isolates: Leptospira interrogans (n=3), Leptospira kirschneri (n=8) and Leptospira borgpetersenii (n=21). MAbs and PFGE characterized pathogenic isolates at the serovar level and resulted mainly in agreement (64%) although many discrepancies (35%) were observed. |
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Keywords: | Portugal Taxonomy Serotyping Genotyping Leptospira |
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