A Bacterial Cell Wall Stain1 |
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Authors: | H. L. Chance |
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Affiliation: | a University of Oklahoma, Norman, Okla |
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Abstract: | A new method is given to stain bacterial cell walls, especially of Escherichia coli and Bacillus cereus. The cells are smeared in water on a slide and, as soon as air-dry, are stained 3-4 minutes with a 1 % aqueous solution of new fuchsin. The smear is washed with water until the stain ceases to run and is then allowed to air dry. The slide is placed on a 50°C. warm plate for 10-20 seconds, and the smear is then covered with a thin film of a 1-2% solution of Congo red at a pH of about 9.5. The smear is ready for observation as soon as dry or it may be washed with water if desired before observation. |
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