首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Decrease in IGF-I binding sites on human promyelocytic leukemia cell line (HL-60) with differentiation
Authors:I Sukegawa  N Hizuka  K Takano  K Asakawa  K Shizume
Institution:Department of Medicine, Tokyo Women's Medical College, Japan.
Abstract:Specific insulin-like growth factor I (IGF-I) receptors on human promyelocytic leukemia cell line (HL-60) were identified and characterized. 125I]IGF-I specifically bound to the cells, and 125I]IGF-I binding to the cells was displaced by unlabeled IGF-I in a dose dependent manner. 125I]IGF-I binding to the cells were displaced by multiplication stimulating activity (MSA) and porcine insulin, with potencies that were 10 and 100 times less than that of IGF-I, respectively. By an affinity labeling technique, IGF type I receptors were found to be present on the HL-60 cells. After the cells were differentiated to the macrophage-like cells by 12-o-tetra-decanoyl-phorbol-13-acetate (TPA) and 1,25-dihydroxy-vitamin D3 (1,25(OH)2D3), 125I]IGF-I binding to the cells decreased significantly. By Scatchard analysis, it was found to be due to a decrease in the number of IGF-I receptors. Thus, the differentiation of HL-60 cells to the macrophage-like cells was accompanied by a decrease in IGF-I receptors.
Keywords:
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号