Studies on zinc- and cadmium-bound proteins in bovine kidneys by biochemical and neutron activation techniques |
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Authors: | Jayawickreme C K Chatt A |
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Institution: | 1.Trace Analysis Research Centre, Department of Chemistry, Dalhousie University, B3H 4J1, Halifax, NS, Canada ; |
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Abstract: | Bovine kidneys were found to contain about 78 ppm Zn and 0.78 ppm Cd. Approximately 45% of Zn and 60% of Cd were present in
the cytosol fraction. More than 95% of these two metals were bound to macromolecules. Both Zn- and Cd-protein complexes were
observed to be stable between pH 7 and 10.5. Separation and characterization of these proteins were carried out using several
chromatographic and electrophoretic techniques in conjunction with instrumental neutron activation analysis (INAA). The results
showed the presence of at least four Zn-binding proteins with mol wt>300,000, 260,000, 89,000, and 27,000 and at least three
Cd-binding proteins of mol wt>300,000, 32,000, and 13,000. |
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Keywords: | Index Entries" target="_blank">Index Entries Zinc cadmium proteins neutron activation chromatography electrophoretic pH dependence bovine kidney |
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