Transfer and replication of RSF1010-derived plasmids in several cyanobacteria of the generaSynechocystis andSynechococcus |
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| Authors: | Pierre Mermet-Bouvier Corinne Cassier-Chauvat Pierre Marraccini Dr Franck Chauvat |
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| Institution: | (1) Service de Biochimie et Génétique Moléculaire, Département de Biologie Cellulaire et Moléculaire, Commissariat à l'Energie Atomique, Centre d'Etudes de Saclay, F91191 Gif sur Yvette Cedex, France;(2) Laboratoire des Protéines Membranaires Transductrices d'énergie URA D1290, Département de Biologie Cellulaire et Moléculaire, Commissariat à l'Energie Atomique, Centre d'Etudes de Saclay, F91191 Gif sur Yvette Cedex, France |
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| Abstract: | RSF1010-derived plasmids are most efficiently transferred by conjugation to the unicellular cyanobacteriaSynechocystis strains sp. PCC6803 and PCC6714 andSynechococcus strains sp. PCC7942 and PCC6301, where they replicate autonomously, even though they contain no cyanobacterial DNA. These results are especially important in the case of the facultative heterotrophic strainSynechocystis PCC6714, which is not transformable Mol Gen Genet 204:185, 1986] |
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