Isolation and characterization of disc-shaped phycobilisomes from the red alga rhodella violacea |
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Authors: | Klaus P Koller W Wehrmeyer H Schneider |
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Institution: | (1) Fachbereich Biologic-Botanik der Philipps-Universität Marburg, Lahnberge, D-3550 Marburg/Lahn, Germany |
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Abstract: | Disc-shaped phycobilisomes were purified from Triton X100 treated cell homogenates of the unicellular marine red alga, Rhodella violacea. Their absorption spectrum had principal maxima at 544 and 568 nm (B-phycoerythrin), 624 nm (C-phycocyanin) and a distinct shoulder at 652 nm (allophycocyanin). Intermolecular energy transfer within the phycobilisomes was clearly demonstrated by fluorescence data. Excited at 546 nm intact phycobilisomes showed a main fluorescence emission maximum at 665 nm, a minor one at 577 nm and a shoulder at 730 nm.Dissociated phycobilisomes revealed a composition of 58% B-phycoerythrin, 25% C-phycocyanin and 17% allophycocyanin under the cultural conditions used. Analytical methods resolved no other components than phycobiliproteins. In addition to the defined C-phycocyanin and two isoproteins of B-phycoerythrin a stable heterogeneous aggregate of B-phycoerythrin/C-phycocyanin was separated in considerable amounts.In the electron microscope negatively stained phycobilisomes appeared as elliptical aggregates having dimensions slightly above the values found in ultrathin sections and a detailed subunit structure. All observations and data suggest a new rhodophytan phycobilisome type in Rhodella violacea.Abbreviations PBS
phycobilisome(s)
- PE
B-phycoerythrin
- PC
C-phycocyanin
- APC
allophycocyanin
- C
concentration (mg/ml)
- E
extinction |
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Keywords: | Phycobilisomes Isolation methods Phycobiliproteins Accessory photosynthetic pigments Rhodella violacea Rhodophyceae |
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