Identification of microRNAs from Plutella xylostella larvae associated with parasitization by Diadegma semiclausum |
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| Authors: | Kayvan Etebari Mazhar Hussain Sassan Asgari |
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| Institution: | 1. Australian Infectious Disease Research Centre, School of Biological Sciences, The University of Queensland, St. Lucia, Brisbane, QLD 4072, Australia;2. School of Biological Sciences, Monash University, Clayton, Victoria 3800, Australia;1. Institute of Evolutionary Biology (CSIC – Universitat Pompeu Fabra), Passeig Maritim de la Barceloneta 37, 0803 Barcelona, Spain;2. Institute for Research in Biomedicine (IRB Barcelona), Baldiri Reixac 10, 08028 Barcelona, Spain;1. Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200032, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;3. Division of Plant Sciences, University of Missouri, Columbia, MO, USA;4. USDA/Agricultural Research Service, Biological Control of Insects Research Laboratory, Columbia, MO, USA;1. Faculty of Life Sciences, Northwestern Polytechnical University, Xi''an 710072, China;2. Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China;3. University of Chinese Academy of Sciences, Beijing 100049, China;4. Division of Plant Sciences, University of Missouri, Columbia, MO, USA |
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| Abstract: | MicroRNAs (miRNAs) as small non-coding RNAs play important roles in many biological processes such as development, cell signaling and immune response. Small RNA deep sequencing technology provided an opportunity for a thorough survey of miRNAs in a global key pest Plutella xylostella as well as comparative analysis of miRNA expression profile of the insect in association with parasitization by Diadegma semiclausum. Combining the deep sequencing data and bioinformatics, 235 miRNAs were identified from P. xylostella. Differential expression of host cellular miRNAs in response to parasitism was examined by making small RNA libraries from parasitized and naive second instar larvae of P. xylostella. Bantam, miR-276*, miR-10, miR-31 and miR-184 were detected as five most abundant miRNAs in both libraries and 96 miRNAs were identified that were differentially expressed after parasitization. Bantam*, miR-184 and miR-281* were significantly down-regulated and two miRNAs miR-279b and miR-2944b* were highly induced in parasitized larvae. Interestingly, high copy numbers and differential expression of several miRNA passenger strands (miRNA*) suggest their potential roles in host-parasitoid interaction. In conclusion, expression profiling of miRNAs provided insights into their possible involvement in insect immune response to parasitism and offer an important resource for further studies. |
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