Remarkable stabilization of antiparallel DNA triplexes by strong stacking effects of consecutively modified nucleobases containing thiocarbonyl groups |
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Authors: | Kenji Yamada Yusaku Hattori Takeshi Inde Takashi Kanamori Akihiro Ohkubo Kohji Seio Mitsuo Sekine |
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Affiliation: | 1. Department of Life Science, Tokyo Institution of Technology, 4259 Nagatsuta, Yokohama 226-8501, Japan;2. Education Academy of Computational Life Science, Tokyo Institution of Technology, 4259 Nagatsuta, Yokohama 226-8501, Japan |
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Abstract: | The consecutive arrangement of 2′-deoxy-6-thioguanosines (s6Gs) and 4-thiothymidines (s4Ts) in antiparallel triplex-forming oligonucleotides (TFOs) considerably stabilized the resulting antiparallel triplexes with high base recognition ability by the strong stacking effects of thiocarbonyl groups. This result was remarkable because chemical modifications of the sugar moieties and nucleobases of antiparallel TFOs generally destabilize triplex structures. Moreover, in comparison with unmodified TFOs, it was found that TFOs containing s6Gs and s4Ts could selectively bind to the complementary DNA duplex but not to mismatched DNA duplexes or single-stranded RNA. |
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