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A convenient synthesis of GDP-d-rhamnose: The donor substrate for d-rhamnosyltransferase WbpZ from Pseudomonas aeruginosa PAO1 |
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| Authors: | Shuo Wang Hidenori Tanaka Ole Hindsgaul Joseph S Lam Inka Brockhausen |
| Institution: | 1. Department of Medicine, Queen’s University, Kingston, Ontario, Canada K7L 3N6;2. Department of Biomedical and Molecular Sciences, Queen’s University, Kingston, Ontario, Canada K7L 3N6;3. Carlsberg Laboratory, Copenhagen-V DK-1799, Denmark;4. Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1 |
| Abstract: | Gram negative bacteria have lipopolysaccharides (LPS) that are critical for their survival. LPS molecules are composed of antigenic exopolysaccharide chains (O antigens). We are interested in discovering the enzymes involved in the biosynthesis of O antigens in Pseudomonas aeruginosa. The common polysaccharide antigen contains α-linked d-rhamnose residues. We have now synthesized GDP-d-rhamnose by a convenient synthesis in aqueous solution, and have shown that it can be used without extensive purification as the donor substrate for d-rhamnosyltransferase (WbpZ) from the P. aeruginosa strain PAO1. The availability of this nucleotide sugar preparation allows for characterization of d-rhamnosyltransferases. |
| Keywords: | O antigens WbpZ |
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