Targeting prostate cancer cells with PSMA inhibitor-guided gold nanoparticles |
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Authors: | Benjamin B. Kasten Tiancheng Liu Jessie R. Nedrow-Byers Paul D. Benny Clifford E. Berkman |
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Affiliation: | 1. Department of Chemistry, Washington State University, Pullman, WA 99164-4630, USA;2. Cancer Targeted Technology, Woodinville, WA 98072, USA |
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Abstract: | Prostate-specific membrane antigen (PSMA) is a notable biomarker for diagnostic and therapeutic applications in prostate cancer. Gold nanoparticles (AuNPs) provide an attractive nanomaterial platform for combining a variety of targeting, imaging, and cytotoxic agents into a unified device for biomedical research. In this study, we present the generation and evaluation of the first AuNP system functionalized with a small molecule phosphoramidate peptidomimetic inhibitor for the targeted delivery to PSMA-expressing prostate cancer cells. The general approach involved the conjugation of streptavidin-coated AuNPs with a biotin-linked PSMA inhibitor (CTT54) to generate PSMA-targeted AuNPs. In vitro evaluations of these targeted AuNPs were conducted to determine PSMA-mediated and time-dependent binding to PSMA-positive LNCaP cells. The PSMA-targeted AuNPs exhibited significantly higher and selective binding to LNCaP cells compared to control non-targeted AuNPs, thus demonstrating the feasibility of this approach. |
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