Formation of 8-S-l-cysteinylguanosine from 8-bromoguanosine and cysteine |
| |
Authors: | Toshinori Suzuki Aya Kosaka Michiyo Inukai |
| |
Institution: | School of Pharmacy, Shujitsu University, Okayama 703-8516, Japan |
| |
Abstract: | When 8-bromoguanosine was incubated with cysteine at pH 7.4 and 37 °C, a previously unidentified product was formed as a major product in addition to guanosine. The product was identified as a cysteine substitution derivative of guanosine at the 8 position, 8-S-l-cysteinylguanosine. The reaction was accelerated under mildly basic conditions. The cysteine adduct of guanosine was fairly stable and decomposed with a half-life of 193 h at pH 7.4 and 37 °C. Similar results were observed for incubation of 8-bromo-2′-deoxyguanosine with cysteine. The results suggest that 8-bromoguanine in nucleosides, nucleotides, RNA, and DNA can react with thiols resulting in stable adducts. |
| |
Keywords: | 8-Bromoguanosine Cysteine Guanosine |
本文献已被 ScienceDirect 等数据库收录! |
|