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Catching HIV ‘in the act’ with 3D electron microscopy
Authors:Lesley A Earl  Jeffrey D Lifson  Sriram Subramaniam
Institution:1. Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA;2. AIDS and Cancer Virus Program, SAIC-Frederick, Inc., Frederick National Laboratory, Frederick, MD 21702, USA;1. Department of Emergency and Critical Care Medicine, Nara Medical University, Kashihara, Nara, Japan;2. Department of Radiology, Nara Medical University, Kashihara, Nara, Japan;3. Laboratory for Experimental Brain Research, Lund University, Lund, Sweden;2. Discovery Technologies, Small Molecule Research, F. Hoffmann-La Roche AG, Basel, Switzerland;3. Cardiovascular & Metabolic Disease Translational Area, Pharma Research & Early Development, F. Hoffmann-La Roche AG, Basel, Switzerland;1. State Key Laboratory for Geo-mechanics and Deep Underground Engineering, China University of Mining and Technology, Xuzhou 221116, China;2. School of Mechanics and Civil Engineering, China University of Mining and Technology, Jiangsu, Xuzhou 221116, China;3. State Key Laboratory of Explosion Science and Technology, Beijing Institute of Technology, Beijing 100081, China;1. Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA;2. Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N., Seattle, WA 98109, USA;3. Department of Global Health, University of Washington, Seattle, WA 98195, USA;4. Department of Immunology & Microbiology, IAVI Neutralizing Antibody Center, Center for HIV/AIDS Vaccine Immunogen Development, The Scripps Research Institute, La Jolla, CA 92037, USA;5. Oregon National Primate Research Center, Oregon Health & Science University, Portland, OR 97006, USA;6. Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA;7. Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA;8. Beth Israel Deaconess Medical Center, Boston, MA 02115, USA;9. Department of Biostatistics, University of Washington, Seattle, WA 98195, USA
Abstract:
Keywords:cryo-electron tomography  focused ion beam scanning electron microscopy (FIB-SEM)  ion abrasion scanning electron microscopy (IA-SEM)  vaccine design  virus–cell interaction
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