Synthesis of DNA oligonucleotides containing C5-ethynylbenzenesulfonamide-modified nucleotides (EBNA) by polymerases towards the construction of base functionalized nucleic acids |
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| Authors: | Astrid Goubet Antoine Chardon Pawan Kumar Pawan K. Sharma Rakesh N. Veedu |
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| Affiliation: | 1. School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Brisbane 4072, Australia;2. Institut Polytechnique LaSalle Beauvais, Beauvais 60000, France;3. Department of Chemistry, Kurukshetra Universiy, Kurukshetra 136119, India |
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| Abstract: | C5-Ethynylbenzenesulfonamide-modified nucleotide (EBNA) was investigated as substrate of various DNA polymerases. The experiments revealed that KOD, Phusion and Klenow DNA polymerases successfully accepted EBNA-T nucleotide as a substrate and yielded the fully extended DNA. KOD DNA polymerase was found to be the most efficient enzyme to furnish EBNA-T containing DNA in good yields. Phusion DNA polymerase efficiently amplified the template containing EBNA-T nucleotides by PCR. |
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