Identification of amides derived from 1H-pyrazolo[3,4-b]pyridine-5-carboxylic acid as potent inhibitors of human nicotinamide phosphoribosyltransferase (NAMPT) |
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Authors: | Xiaozhang Zheng Kenneth W Bair Paul Bauer Timm Baumeister Krista K Bowman Alexandre J Buckmelter Maureen Caligiuri Karl H Clodfelter Yezhen Feng Bingsong Han Yen-Ching Ho Nikolai Kley Hong Li Xiaorong Liang Bianca M Liederer Jian Lin Justin Ly Thomas O’Brien Peter S Dragovich |
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Institution: | 1. Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA;2. Forma Therapeutics, Inc., 500 Arsenal Street, Watertown, MA 02472, USA;3. Pharmaron Beijing, Co. Ltd, 6 Taihe Road, BDA, Beijing 100176, PR China |
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Abstract: | Potent, 1H-pyrazolo3,4-b]pyridine-containing inhibitors of the human nicotinamide phosphoribosyltransferase (NAMPT) enzyme were identified using structure-based design techniques. Many of these compounds exhibited nanomolar antiproliferation activities against human tumor lines in in vitro cell culture experiments, and a representative example (compound 26) demonstrated encouraging in vivo efficacy in a mouse xenograft tumor model derived from the A2780 cell line. This molecule also exhibited reduced rat retinal exposures relative to a previously studied imidazo-pyridine-containing NAMPT inhibitor. Somewhat surprisingly, compound 26 was only weakly active in vitro against mouse and monkey tumor cell lines even though it was a potent inhibitor of NAMPT enzymes derived from these species. The compound also exhibited only minimal effects on in vivo NAD levels in mice, and these changes were considerably less profound than those produced by an imidazo-pyridine-containing NAMPT inhibitor. The crystal structures of compound 26 and the corresponding PRPP-derived ribose adduct in complex with NAMPT were also obtained. |
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Keywords: | Nicotinamide phosphoribosyltransferase (NAMPT) X-ray crystal structure Tumor metabolism |
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