Active site characterization and molecular cloning of Tenebrio molitor midgut trehalase and comments on their insect homologs |
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| Authors: | Ana Gomez Christiane Cardoso Fernando A. Genta Walter R. Terra Clélia Ferreira |
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| Affiliation: | 1. Institute of Physiology, Academy of Sciences of the Czech Republic v.v.i., Videnska 1083, 14220 Prague, Czech Republic;2. 2nd Faculty of Medicine, Charles University, V Uvalu 84, 150 06 Prague, Czech Republic;3. Institute of Microbiology, Academy of Sciences of the Czech Republic v.v.i., Videnska 1083, 14220 Prague, Czech Republic;4. Department of Biochemistry, Faculty of Science, Charles University, Hlavova 2030, 12843 Prague, Czech Republic;5. Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University, Hlavova 2030, 12843 Prague, Czech Republic |
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| Keywords: | Trehalase Multiple inhibition analysis Chemical modification Active groups Trehalase evolution DPC" },{" #name" :" keyword" ," $" :{" id" :" kwrd0040" }," $$" :[{" #name" :" text" ," _" :" diethylpyrocarbonate EDC" },{" #name" :" keyword" ," $" :{" id" :" kwrd0050" }," $$" :[{" #name" :" text" ," _" :" N- (3-dimethylaminopropyl)-N″- ethylcarbodiimide EDTA" },{" #name" :" keyword" ," $" :{" id" :" kwrd0060" }," $$" :[{" #name" :" text" ," _" :" ethylenediaminetetraacetic acid HEPES" },{" #name" :" keyword" ," $" :{" id" :" kwrd0070" }," $$" :[{" #name" :" text" ," _" :" 4-(2- hydroxyethyl)-1-piperazineethanesulfonic acid NBS" },{" #name" :" keyword" ," $" :{" id" :" kwrd0080" }," $$" :[{" #name" :" text" ," _" :" N-bromosuccinimide or- laminarin" },{" #name" :" keyword" ," $" :{" id" :" kwrd0090" }," $$" :[{" #name" :" text" ," _" :" periodate-oxidized and reduced laminarin PG" },{" #name" :" keyword" ," $" :{" id" :" kwrd0100" }," $$" :[{" #name" :" text" ," _" :" 1 phenylglyoxal pHMB" },{" #name" :" keyword" ," $" :{" id" :" kwrd0110" }," $$" :[{" #name" :" text" ," _" :" 4-(hydroxymercuri) benzoic acid TEMED" },{" #name" :" keyword" ," $" :{" id" :" kwrd0120" }," $$" :[{" #name" :" text" ," _" :" N, N, N″, N″- tetramethylethylenediamine TNM" },{" #name" :" keyword" ," $" :{" id" :" kwrd0130" }," $$" :[{" #name" :" text" ," _" :" tetranitromethane |
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