Active site characterization and molecular cloning of Tenebrio molitor midgut trehalase and comments on their insect homologs期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Active site characterization and molecular cloning of Tenebrio molitor midgut trehalase and comments on their insect homologs

Authors:	Ana Gomez Christiane Cardoso Fernando A Genta Walter R Terra Clélia Ferreira

Institution:	1. Institute of Physiology, Academy of Sciences of the Czech Republic v.v.i., Videnska 1083, 14220 Prague, Czech Republic;2. 2nd Faculty of Medicine, Charles University, V Uvalu 84, 150 06 Prague, Czech Republic;3. Institute of Microbiology, Academy of Sciences of the Czech Republic v.v.i., Videnska 1083, 14220 Prague, Czech Republic;4. Department of Biochemistry, Faculty of Science, Charles University, Hlavova 2030, 12843 Prague, Czech Republic;5. Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University, Hlavova 2030, 12843 Prague, Czech Republic

Abstract:

Keywords:	Trehalase Multiple inhibition analysis Chemical modification Active groups Trehalase evolution DPC"} {"#name":"keyword" "$":{"id":"kwrd0040"} "$$":[{"#name":"text" "_":"diethylpyrocarbonate EDC"} {"#name":"keyword" "$":{"id":"kwrd0050"} "$$":[{"#name":"text" "_":"N- (3-dimethylaminopropyl)-N″- ethylcarbodiimide EDTA"} {"#name":"keyword" "$":{"id":"kwrd0060"} "$$":[{"#name":"text" "_":"ethylenediaminetetraacetic acid HEPES"} {"#name":"keyword" "$":{"id":"kwrd0070"} "$$":[{"#name":"text" "_":"4-(2- hydroxyethyl)-1-piperazineethanesulfonic acid NBS"} {"#name":"keyword" "$":{"id":"kwrd0080"} "$$":[{"#name":"text" "_":"N-bromosuccinimide or- laminarin"} {"#name":"keyword" "$":{"id":"kwrd0090"} "$$":[{"#name":"text" "_":"periodate-oxidized and reduced laminarin PG"} {"#name":"keyword" "$":{"id":"kwrd0100"} "$$":[{"#name":"text" "_":"1 phenylglyoxal pHMB"} {"#name":"keyword" "$":{"id":"kwrd0110"} "$$":[{"#name":"text" "_":"4-(hydroxymercuri) benzoic acid TEMED"} {"#name":"keyword" "$":{"id":"kwrd0120"} "$$":[{"#name":"text" "_":"N N N″ N″- tetramethylethylenediamine TNM"} {"#name":"keyword" "$":{"id":"kwrd0130"} "$$":[{"#name":"text" "_":"tetranitromethane
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