| Abstract: | With water as sole eluant, the retention volumes for carbohydrates on an aluminate-resin column generally decrease in the order: ketoses>aldoses>alditols>methyl glycosides; retention increases with molecular size. Both aluminate ion and hydroxide ion contribute to the chromatographic properties of an aluminate resin. To avoid alkaline degradation and interconversion, which can occur extensively at 25°, chromatography of reducing sugars must be performed both rapidly and at low temperature. A mixture of d-glucose and d-fructose can be completely separated on a short aluminate column. |
