Direct cell penetration of the antifungal peptide,MMGP1, in Candida albicans |
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Authors: | Muthuirulan Pushpanathan Jeyaprakash Rajendhran Sathyanarayanan Jayashree Balakrishnan Sundarakrishnan Seetharaman Jayachandran Paramasamy Gunasekaran |
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Affiliation: | 1. Department of Genetics, Centre for Excellence in Genomic Sciences, School of Biological Sciences, Madurai Kamaraj University, , Madurai, 625 021 India;2. Department of Biotechnology, School of Life Sciences, Pondicherry University, , Pondicherry, 605 014 India |
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Abstract: | An antifungal peptide, MMGP1, was recently identified from marine metagenome. The mechanism of cellular internalization of this peptide in Candida albicans was studied using fluorescein 5–isothiocynate (Sigma, California, USA) labeling followed by fluorescence microscopy and flow cytometry analyses. The peptide could enter C. albicans cells even at 4 °C, where all energy‐dependent transport mechanisms are blocked. In addition, the peptide internalization was not affected by the endocytic inhibitor, sodium azide. The kinetic study has shown that the peptide was initially localized on cell membrane and subsequently internalized into cytosol. The MMGP1 treatment exhibited time‐dependent cytotoxicity in C. albicans as evidenced by SYTOX Green (Molecular Probes Inc., Eugene, Oreg) uptake. Copyright © 2012 European Peptide Society and John Wiley & Sons, Ltd. |
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Keywords: | marine metagenome antifungal peptide cell penetration fungicidal effect |
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