The nature of radiation-induced mutations at the white locus of Drosophila melanogaster |
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| Authors: | A Pastink A P Schalet C Vreeken E Parádi J C Eeken |
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| Affiliation: | 1. Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, Wassenaarseweg 72, Leiden, The Netherlands;2. J.A. Cohen Institute, Interuniversity Institute for Radiopathology and Radiation Protection, Leiden, The Netherlands;3. Department of Genetics, Eötvös Loránd University, Múzéum krt. 4/a, H-1088 Budapest, Hungary;1. Institute of Molecular Genetics, Russian Academy of Sciences, Kutchatov Sq. 2, Moscow, 123182, Russia;2. Moscow State University of Education, M. Pirogovskaya Str. 1/1, Moscow, 119991, Russia;3. Skolkovo Institute of Science and Technology, Nobelya Ulitsa 3, Moscow, 121205, Russia;4. Institute for Information Transmission Problems, Russian Academy of Sciences, Bolshoy Karetny per., h. 19, b. 1, Moscow, 127994, Russia;5. Petersburg Nuclear Physics Institute named by B.P. Konstantinov of NRC «Kurchatov Institute», Orlova Roscha, Gatchina, 1888300, Russia;6. N. M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Kosygin St. 4, Moscow, 119334, Russia;7. Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, 4, Oparina street, Moscow 117997, Russia;8. Belozerskii Institute of Physico-Chemical Biology, Moscow State University, Leninskie gory 1, Moscow 119992, Russia;9. Moscow Institute of Physics and Technology, Institutskii Per. 9, Dolgoprudny, Moscow Region, 141700, Russia;10. Centre for Cognition and Decision Making, Institute for Cognitive Neuroscience, National Research University Higher School of Economics, Russian Federation, Krivokolenniy sidewalk, 3a, Moscow, 101000, Russia;1. Department of General and Medical Biochemistry, Faculty of Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland;2. Department of Theoretical Chemistry, Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland;1. Department of Molecular and Cellular Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA;2. Department of Medical Microbiology and Immunology, and Program in Bioinformatics, The University of Toledo College of Medicine and Life Sciences, Toledo, OH 43614, USA;1. Department of Cellular, Computational and Integrative Biology (CIBIO), University of Trento, Trento, TN 38123, Italy |
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| Abstract: | X-Ray- and neutron-induced mutations at the white locus of Drosophila melanogaster were used to study the nature of radiation-induced genetic damage. Genetic analysis showed the presence of multi-locus deficiencies in 15 out of 31 X-ray mutants and in 26 out of 35 mutants induced by neutrons. The DNA from 11 X-ray and 4 neutron mutants, which were not multi-locus deficiencies, was analyzed by Southern blot-hybridization. Deletions were observed in 2 X-ray and 1 neutron mutant. In combination with cytogenetic techniques, chromosomal rearrangements affecting the white locus (translocations, inversions, etc.) were identified in 3 X-ray and in 2 neutron mutants. A hot-spot for translocation breakpoints was identified in the left arm of the third chromosome. 5 X-ray mutants, which apparently did not contain large deletions, were subjected to further analysis by the nuclease S1 protection method, after cloning of the white gene. In 4 mutants a small deletion could indeed be detected in this way. Thus it seems that by far the main part of X-ray- and neutron-induced white mutants have arisen through large changes in the white gene, especially deletions. |
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