Production of peptide leukotrienes in endotoxin shock |
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Authors: | Wolfgang Hagmann Claudio Denzlinger Dietrich Keppler |
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Affiliation: | Biochemisches Institut, Universität Freiburg, Hermann-Herder-Str. 7, D-7800 Freiburg i. Br., FRG |
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Abstract: | Arachidonate metabolites are potent mediators generated in endotoxin shock. Following endotoxin administration (15 mg/kg) into unanesthetized rats, we found a rapid biliary secretion of peptide leukotrienes. Analysis of bile for peptide leukotrienes included organic solvent extractions, reversed phase-HPLC, radioimmunoassay (RIA), and spectrophotometry. The major immunoreactive endogenous leukotriene (LT) from bile was eluted between LTC4 and LTD4 in three chromatographic systems. It corresponded thereby to a biliary metabolite of injected LTC4 and LTD4 which in turn showed the ultraviolet spectrum of a peptide leukotriene. This demonstration of endotoxin-induced generation of peptide LTs in vivo was possible by sequential HPLC and RIA analyses in bile into which peptide LTs are eliminated from blood. |
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Keywords: | Leukotriene Endotoxin Bile Radioimmunoassay HPLC Eicosanoid |
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