A fluorescence-based Coomassie Blue protocol for two-dimensional gel-based proteomics |
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| Authors: | Yongxin Yang Jiaqi Wang Dengpan Bu Leying Zhang Shanshan Li Lingyun Zhou Hongyang Wei |
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| Affiliation: | (1) State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2, Yuan Ming Yuan West Road, Haidian District, Beijing, 100193, China;(2) Institute of Animal Science, Anhui Academy of Agricultural Sciences, Hefei, 230031, China; |
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| Abstract: | A sensitive and convenient “visible SYPRO” staining protocol was developed for visualizing proteins after SDS-PAGE. Gels were sensitized with SYPRO Ruby and then stained with the Coomassie Brilliant Blue G-250 protocol (Blue Silver). This combined protocol had similar or better linearity than staining with only SYPRO Ruby or Blue Silver, respectively. In addition, this method was more sensitive than that of Blue Silver, simpler than that of SYPRO Ruby, and compatible with subsequent mass spectrometry analysis. |
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