Somatic embryogenesis in Sitka spruce (Picea sitchensis (Bong.) Carr.) |
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| Authors: | Peter Krogstrup Erik N. Eriksen Jette D. Møller Hans Roulund |
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| Affiliation: | (1) Botanic Garden, Cell & Tissue Culture Section, University of Copenhagen, Øster Farimagsgade 2 B, 1353 K, Denmark;(2) Department of Horticulture, The Royal Veterinary and Agricultural University of Copenhagen, Rolighedsvej 23, 1870 Frederiksberg C, Denmark;(3) Botanic Laboratory, University of Copenhagen, Gothersgade 140, 1123 K, Denmark;(4) The Royal Veterinary and Agricultural University of Copenhagen, The Arboretum, 2970 Hørsholm, Denmark |
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| Abstract: | Embryonal-suspensor masses from immature embryos from cones of Sitka spruce (Picea sitchensis (Bong.) Carr.) proliferated on a modified Murashige & Skoog medium with N6-benzyl-aminopurine, kinetin, 2,4-dichlorophenoxyacetic acid and an organic nitrogen source. The slimy white embryonal-suspensor masses with proembryos were maintained on a solid proliferation medium with reduced amounts of growth regulators. Transfer of embryonal-suspensor masses to a non-woven polyester carrier with liquid maturation media containing ±2-cis-4-trans-abscisic acid and a reduced amount of inositol and organic nitrogen resulted in synchronized embryo formation. Further development was achieved on a medium without ±2-cis-4-trans-abscisic acid and organic nitrogen. Somatic embryos were successfully transferred ex vitrum.Abbreviations ABA ±2-cis-4-trans-abscisic acid - BAP N6-benzyl-aminopurine - ESM embryonal-suspensor masses - KIN kinetin - 2,4-D 2,4-dichlorophenoxyacetic acid |
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