Cryopreservation of in vitro sugar beet shoot tips using the encapsulation-dehydration technique: influence of abscisic acid and cold acclimation |
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Authors: | B Vandenbussche M P De Proft |
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Institution: | (1) Faculty of Agricultural and Applied Biological Sciences, Katholieke Universiteit Leuven, Willem de Croylaan 42, B-3001 Heverlee, Belgium e-mail: Bert.Vandenbussche@agr.kuleuven.ac.be Fax: +32-16-322966, BE |
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Abstract: | It has been previously shown that shoot tips of in vitro plantlets of sugar beet (Beta vulgaris L. clone SES1) can be cryopreserved using the encapsulation-dehydration technique (survival rate of 37% after freezing).
This article reports the influence of abscisic acid (ABA) and cold acclimation on survival after cryopreservation. When ABA
was added to the multiplication medium of the plants, the survival rate of shoot tips after cryopreservation was not increased
(45%). After cold acclimation of the plants, their growth pattern differed (plants became apically dominant) and the survival
rate of the shoot tips after cryopreservation clearly increased (70% survival and 50% plant regeneration after freezing).
This improved protocol was successfully applied to three other clones.
Received: 28 October 1996 / Revision received: 28 January 1997 / Accepted: 15 March 1997 |
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Keywords: | Beta vulgaris L Cryopreservation Encapsulation-dehydration technique Abscisic acid Cold acclimation |
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