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Oligomeric organization of recombinant human neurotrophins expressed in Escherichia coli cells |
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| Authors: | Safina D R Rafieva L M Koval' A V Shkurina E E Dmitrieva V G Raevskaia N M Gasanov E V Demidiuk I V Kostrov S V |
| Abstract: | Genes of human neurotrophins NGF, BDNF, NT-3 were cloned, and the corresponding proteins and their fragments were expressed in Escherichia coli BL-21 (DE3lambda) cells. Their intracellular localization was determined. The conditions for isolation and purification of the target recombinant proteins and for folding of BDNF and NT-3 precursors were selected. The recombinant proprecursors of human neurotrophines have been shown to possess complex oligomeric structure. |
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