盐胁迫浓度与大麦幼苗生长分裂和遗传损伤的相关性研究

研究盐胁迫浓度与大麦幼苗生长分裂的关系, 实验结果表明:大麦幼苗生长在一定浓度的NaCl 溶液中时, 幼苗生长抑制、叶绿素含量降低, 有丝分裂指数下降, 根尖分生区细胞中具有微核和染色体畸变的细胞明显增多。统计分析结果显示:幼苗的分裂指数、遗传损伤与盐浓度间有很好的线性关系, 有丝分裂指数与处理浓度间呈负相关(p < 0.01), 微核率、染色体畸变率两项指标与处理浓度间呈正相关(p < 0.01)。研究结果表明:大麦幼根有丝分裂指数、微核率及染色体畸变率可以作为监测环境NaCl 的定量指标。

ON RELATIONSHIP OF SODIUM CHLORIDE CONCENTRATION TO CELL DIVISION,GENETIC DAMAGE AND GROWTH OF HORDEUM VULGARE SEEDLINGS

The effects of NaCl solution on cell division and growth of Hordeum vulgare seedlings were studied. Seedlings' chlorophyll content was lower than the control's when they incubated in NaCl solution. The seedlings grew slowly and their mitotic index (MI) decreased significantly when seedlings incubated at high concentration. The frequencies of micronuclei (MN) and chromosomal aberration (CA) increased in root tip cells of Hordeum vulgare seedlings at the same time. The results of statistics showed that there was a linear function between NaCl concentration and MI (also MN and CA). Mitotic index showed negative responses to NaCl concentration. The concentration of NaCl solution showed positive responses both in micronucleus and chromosomal aberration. It suggested that MI, MN and CA of mitosis in Hordeum vulgare seedlings could be used to monitoring environmental sodium chloride.