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Iridoid and caffeoyl phenylethanoid glycosides of the endangered carnivorous plant Pinguicula lusitanica L. (Lentibulariaceae) |
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| Authors: | Toms Grevenstuk Justin JJ van der Hooft Jacques Vervoort Pieter de Waard Anabela Romano |
| Institution: | aIBB-Institute for Biotechnology and Bioengeneering, Faculty of Sciences and Technology, University of Algarve, Campus of Gambelas, Ed. 8, 8005-139 Faro, Portugal;bLaboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703 HA Wageningen, The Netherlands;cWageningen NMR Center, Wageningen University, Dreijenlaan 3, 6703 HA, Wageningen, The Netherlands |
| Abstract: | This work reports for the first time the identification of the major compounds of Pinguicula lusitanica, an endangered carnivorous plant species, using minimal amounts of plant material. A methanol extract was prepared from in vitro cultured plantlets and analyzed by HPLC–SPE–NMR/HPLC–MS. Three iridoid and five caffeoyl phenylethanoid glycosides were identified. These groups of natural compounds were previously reported in the Lentibulariaceae family and have been used as chemotaxonomic markers in related families. |
| Keywords: | Lamiales Globularin Verbascoside Hyphenated techniques LC– MS HPLC– SPE– NMR |
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