A nondestructive method for the measurement of radioactive 14CO2 in blood |
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| Authors: | F J Casado J A Fernández-López J M Argilés M Alemany |
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| Institution: | Departamento de Bioquímica y Fisiologia, Universidad de Barcelona, Spain. |
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| Abstract: | A method for estimation of 14CO2 present in blood and tissular samples is described. It is basically based on the introduction of large amounts of a gas mixture (95% O2, 5% CO2) in the samples which serves to remove the CO2 label by gas dilution. The gas phase is later captured in scintillation vials containing an organic-soluble base that retains the carbon label. The results obtained by means of this methodology show much better recoveries for blood samples than those obtained when the classic acid-diffusion method is used. In addition, it is a very fast procedure which does not alter the pH or protein integrity of the biological sample. |
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