A modified hepatitis B surface antigen carrying both preS1 and preS2 epitopes |
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Authors: | HUI Jingyi LI Guangdi KONG Yuying WANG Yuan |
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Institution: | (1) Shanghai Institute of Biochemistry, Chinese Academy of Sciences, 200031 Shanghai, China |
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Abstract: | The DNA fragments coding for preS2(120–146) and preS1(21–47) amplified by PCR were fused to both 5′ and 3′ ends of S gene
at the position of amino acid 223. The fusion gene was placed downstream of the promoter P7.5 of the universal vaccinia viral
vector pGJP-5 and the recombinant vaccinia virus vS2SSI was then selected by invivo homogeneous recombination. Fusion protein S2SS1 could be expressed in the mammalian cells infected with vS2SSl. The investigation
of expression, secretion, antigenicity and particle assembly of the S2SS1 protein demonstrated that S2SS1 protein could be
assembled into particles which presented preSl, preS2 and S antigenicity and be efficiently secreted from the cells. It also
showed that the level of its expression and secretion approached to that of the S protein expressed by the recombinant vaccinia
virus.
Project supported by the National “863” High Technology Foundation of China. |
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Keywords: | hepatitis B surface antigen (HBsAg) preS1 and preS2 epitopes fusion protein particle recombi nant vaccinia virus |
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