V-1, a catecholamine biosynthesis regulatory protein,positively controls catecholamine secretion in PC12D cells |
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Authors: | Yamakuni Tohru Yamamoto Toshifumi Ishida Yukisato Yamamoto Hideko Song Si Young Adachi Eijiro Hiwatashi Yusuke Ohizumi Yasushi |
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Institution: | Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba-ku, Sendai 980-8578, Japan. yamakuni@mail.pharm.tohoku.ac.jp |
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Abstract: | Stably transfected PC12D cell lines overexpressing a catecholamine biosynthesis regulatory protein, V-1, were used to examine the functional role of V-1 in catecholamine secretion. High K+-induced dopamine secretion in V-1 overexpressing clones was shown to be markedly potentiated compared with control clones carried with a vector alone. As assayed intracellular calcium concentration (Ca2+]i) using fura-PE3, V-1 overexpression was observed to enhance high K+-elicited Ca2+]i elevation. Electron microscopic analysis revealed an increase in dense-cored vesicle formation by V-1 overexpression. These results suggest that the enhancement of high K+-induced dopamine secretion by V-1 overexpression results from the potentiation of high K+-induced Ca2+]i elevation and the increase in the number of dense-cored vesicles. |
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Keywords: | V-1 Overexpression PC12D cells Dopamine biosynthesis Dopamine secretion Exocytosis |
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