Establishment and functional characterization of a tracheal epithelial cell line RTEC11 from transgenic rats harboring temperature-sensitive simian virus 40 large T-antigen |
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Authors: | Tabuchi Yoshiaki Doi Takeshi Takasaki Ichiro Takahashi Ri-ichi Ueda Masatsugu Suzuki Yoshihisa Obinata Masuo |
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Institution: | Division of Molecular Genetics Research, Life Science Research Center, University of Toyama, Toyama, Japan. ytabu@cts.u-toyama.ac.jp |
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Abstract: | A tracheal epithelial cell line RTEC11 was established from transgenic rats harboring temperature-sensitive simian virus 40 large T-antigen. The cells grew continuously at a permissive temperature of 33 degrees C but not at a non-permissive temperature of 39 degrees C. Morphological and functional investigations demonstrated that the cells were polarized epithelial cells maintaining a regulated permeability barrier function. Interestingly, the expression levels of Muc1 (mucin 1) and Scgb1a1 (uteroglobin), non-ciliated secretory cell markers, and Tubb4 (tubulin beta 4), a ciliated cell marker, were significantly increased under the cell growth-restricted condition. Global gene expression and computational network analyses demonstrated a significant genetic network associated with cellular development and differentiation in cells cultured at the non-permissive temperature. The tracheal epithelial cell line RTEC11 with unique characteristics should be useful as an in vitro model for studies of the physiological functions and gene expression of tracheal epithelial cells. |
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Keywords: | Tracheal epithelial cell Temperature‐sensitive SV40 large T‐antigen Non‐ciliated secretory cell Genetic network |
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