Purification and characterization of calmodulin from rat liver mitochondria |
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Authors: | O Hatase M Tokuda T Itano H Matsui A Doi |
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Institution: | Department of Physiology, Kagawa Medical School 1750 Ikenobe, Miki-Town, Kagawa 761-07, Japan |
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Abstract: | Mitochondrial calmodulin of rat liver was purified and classified. It co-migrated with bovine brain calmodulin in non-denaturing polyacrylamide gel electrophoresis, SDS-polyacrylamide gel electrophoresis and isoelectric focusing. The mitochondrial calmodulin activated Ca2+-dependent phosphodiesterase of bovine brain in the presence of Ca2+. About 80% of the mitochondrial calmodulin was proved to be of cytosol origin. It was easily detached by washing with buffer containing EGTA. The other 20% was intramitochondrial calmodulin; half of it was in the matrix space, and half in the membrane. |
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Keywords: | EGTA ethylene glycol bis(β-aminoethylether) N N N′ N′-tetraacetic acid |
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