Localization of five new Ly49 genes, including three closely related to Ly49c |
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Authors: | Karina L McQueen J Douglas Freeman Fumio Takei D L Mager |
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Institution: | (1) Terry Fox Laboratory, British Columbia Cancer Agency, 601 West 10th Avenue, Vancouver, BC V5Z 1L3, Canada, CA;(2) Department of Medical Genetics, University of British Columbia, Vancouver, BC, Canada, CA;(3) Department of Pathology, University of British Columbia, Vancouver, BC, Canada, CA |
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Abstract: | Nine genes belonging to the mouse Ly49 multigene family of natural killer cell receptors have been identified to date. Two of these genes, Ly49h and i, are very closely related to the well characterized Ly49c gene in the carbohydrate recognition domain. Here we show by Southern blotting that at least two additional new sequences
exist in C57BL/6 mice that are also closely related to Ly49c in the carbohydrate recognition domain. Furthermore, in contrast to Ly49a, extensive variation in the arrangement and number of Ly49c–related genes in different mouse strains was observed. To characterize and localize the new Ly49c–related genes in C57BL/6 mice, we isolated and mapped genomic P1 clones hybridizing to an Ly49C exon 7 probe. Locations and the relative order of all Ly49 genes found within the clones was determined. We also used polymerase chain reaction to sequence exons 2, 4, and 7 from all
genes. In this manner, we identified five new potential Ly49 genes which have been tentatively termed Ly49j-n. Ly49j, k, and n belong to the Ly49c–related subfamily, whereas Ly49l and Ly49m are most similar to Ly49d and g, respectively. Interestingly, the members of the Ly49c–related subfamily are not clustered as a unit but are interspersed among other Ly49 genes. These results illustrate the complex nature of the Ly49 gene family and should aid in the understanding of functions, such as the mediation of hybrid resistance, in which Ly49c–related genes play a role.
Received: 10 December 1997 · Revised: 28 February 1998 |
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Keywords: | Natural killer cells Ly49 Multigene family Genomic organization |
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