Germline and somatic mosaicism in transgenic mice |
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| Authors: | T M Wilkie R L Brinster R D Palmiter |
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| Affiliation: | 1. Howard Hughes Medical Institute, Department of Biochemistry SL-15, University of Washington, Seattle, Washington 98195 USA;2. Laboratory of Reproductive Physiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104 USA;1. Department of Allied Health Sciences, University of Connecticut, Storrs, CT, United States;2. Department of Physiology, College of Basic Medical Science, China Medical University, Shenyang, Liaoning, China;3. Fujian Institute of Hematology, Hematology Department of Fujian Medical University Union Hospital, China;4. University of Connecticut Stem Cell Institute, University of Connecticut, Storrs, CT, United States;1. Institute of Rheumatology, Prague, Czech Republic;2. Department of Rheumatology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic;3. 1st Orthopaedic Clinic, 1st Faculty of Medicine, Charles University, Prague, Czech Republic;1. Institute of Psychology, Health & Society, University of Liverpool, Liverpool, United Kingdom;2. Liverpool Ocular Oncology Centre, Royal Liverpool and Broadgreen University Hospital, NHS Trust, Liverpool, United Kingdom;3. Ocular Oncology Service, University of California, San Francisco, San Francisco, California |
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| Abstract: | Analysis of 262 transgenic mouse pedigrees suggests that about 30% of the mice produced by microinjection of plasmids into pronuclei are mosaic in the germline. This implies that in these lines integration of the foreign DNA occurred after the first round of chromosomal DNA replication. In mosaics resulting from delayed integration the transgenic cells are usually distributed to both the trophectoderm and the inner cell mass, but sometimes to only one of these two cell types. Mosaicism of the inner cell mass results in even representation among the somatic tissues, and usually the germline as well; however, the germline is sometimes deficient in or entirely lacks transgenic cells. The germline precursor pool is distinct from the somatic precursor pools; apparently it is either determined prior to the primary germ layers or it is initially composed of fewer cells. |
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