| PCR在猴B病毒与猴病毒8型鉴别中的应用 |
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| 引用本文： | 蔺会云,田克恭,遇秀玲,李敬云,鲍作义,吴娜. PCR在猴B病毒与猴病毒8型鉴别中的应用[J]. 中国实验动物学报, 2001, 9(2): 119-122 |
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| 作者姓名： | 蔺会云 田克恭 遇秀玲 李敬云 鲍作义 吴娜 |
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| 作者单位： | 军事医学科学院实验动物中心,北京 100071 |
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| 基金项目： | 北京市科委实验动物专项基金资助课题!(953 990 70 0 ,954982 60 0 ) |
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| 摘 要： | 目的为了使BV147与SA8相区别,并进一步分析BV147基因结构特点.方法用PCR方法扩增BV147DNA,并对扩增片段进行克隆测序.结果该序列与美国BVE2490株部分基因(UL27)相对应位置的核苷酸同源性为99.54%,与SA8相对应位置的核苷酸同源性为89.91%.结论进一步证明了新分离物为B病毒.
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| 关 键 词： | B病毒 单纯疱疹病毒 聚合酶链反应 核苷酸序列测定 |
| 文章编号： | 1005-4847(2001)02-0119-04 |
| 修稿时间： | 2000-08-02 |
Distinguishing B Virus from Simian Agent 8 by PCR |
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| LIN Huiyun,TIAN Kegong,YU Xiuling,LI Jingyun,BAO Zuoyi,WU Na. Distinguishing B Virus from Simian Agent 8 by PCR[J]. Acta Laboratorium Animalis Scientia Sinica, 2001, 9(2): 119-122 |
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| Authors: | LIN Huiyun TIAN Kegong YU Xiuling LI Jingyun BAO Zuoyi WU Na |
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| Abstract: | Objective To further identify the nature of newly isolated virus,BV147.Methods PCR was used for B virus DNA and the PCR products were sequenced.Results The partial gene of newly isolated B virus showed 99.54% nucleotide identity over the corresponding region of strain E2490 of B virus in America,while only 89.91% over that of SA8.Conclusion The newly isolated culture was demonstrated to be B virus.; |
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| Keywords: | B virus Herpes simplex virus Polymerase chain reaction DNA sequencing |
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